Generator

Part:BBa_K4442000:Design

Designed by: Hala Mahdi   Group: iGEM22_McMaster_Canada   (2022-10-09)


Indole Inducible System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 154
    Illegal NgoMIV site found at 212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504


Design Notes

Something that other teams may want to consider is the length of this part. Along with the other genes we added for outputs (not included here as they are not relevant to the indole sensor), our final block length was longer than 3000bps, meaning that we had to split it up into two sequences to order it from IDT. This complicated our design and building process as we had to manually assemble the two halves of the sequence using PCR, which was quite a finicky process.


Source

This part was first assembled and reported on in this study: Matulis P, Kutraite I, Augustiniene E, Valanciene E, Jonuskiene I, Malys N. Development and Characterization of Indole-Responsive Whole-Cell Biosensor Based on the Inducible Gene Expression System from Pseudomonas putida KT2440. International Journal of Molecular Sciences. 2022; 23(9):4649. https://doi.org/10.3390/ijms23094649


References